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rat monoclonal anti mouse cd93  (R&D Systems)


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    R&D Systems rat monoclonal anti mouse cd93
    Rat Monoclonal Anti Mouse Cd93, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+monoclonal+anti+mouse+cd93/Mouse+C1qR1%2FCD93+Antibody/us08362224-798-18-24
    Average 90 stars, based on 5 article reviews
    rat monoclonal anti mouse cd93 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Saline:

    Article Title: A novel CD93 polymorphism in non-obese diabetic (NOD) and NZB/W F1 mice is linked to a CD4+ iNKT cell deficient state
    Article Snippet: .. Briefly, Immulon 2 HB plates (Thermo Labsystems, Franklin, MA) were coated overnight at 4°C with 2 mcg/ml rat monoclonal anti-mouse CD93 (mAB1696, R&D Systems, Minneapolis, MN) in 0.1 M carbonate buffer, pH 7.4, washed with phosphate buffer saline containing 0.05% Tween-20 (PBST), and blocked with PBST containing 3% dried milk (block buffer) for 1 h. Diluted serum samples were added to wells and incubated for 1 h. After washing with PBST, 1 mcg/ml sheep anti-mouse CD93 antibody (AF1696, R&D Systems) was added to wells for 1 h followed by incubation with anti-sheep horseradish peroxidase-conjugated streptavidin (Jackson, Immunoresearch, West Grove, PA) diluted 1:2,000 in 1% dried milk. .. Sigma fast o -phenylenediamine dihydrochloride peroxidase substrate (Sigma-Aldrich, St. Louis, MO) was added, and the change in absorbance at 405 nm was measured.

    Article Title: Screening for CD93 (C1qRp)-associated polymorphism(S) in the diagnosis, prevention and treatment of autoimmune diseases
    Article Snippet: .. Briefly, Immulon 2 HB plates (Thermo Labsystems, Franklin, Mass.) were coated overnight at 4° C. with 2 □g/ml rat monoclonal anti-mouse CD93 (mAB 1696, R&D Systems, Minneapolis, Minn.) in 0.1 M carbonate buffer, pH 7.4, washed with phosphate buffer saline (PBS) containing 0.05% Tween-20 (PBST), and blocked with PBST containing 3% dried milk (block buffer) for 1 hr. ..

    Blocking Assay:

    Article Title: A novel CD93 polymorphism in non-obese diabetic (NOD) and NZB/W F1 mice is linked to a CD4+ iNKT cell deficient state
    Article Snippet: .. Briefly, Immulon 2 HB plates (Thermo Labsystems, Franklin, MA) were coated overnight at 4°C with 2 mcg/ml rat monoclonal anti-mouse CD93 (mAB1696, R&D Systems, Minneapolis, MN) in 0.1 M carbonate buffer, pH 7.4, washed with phosphate buffer saline containing 0.05% Tween-20 (PBST), and blocked with PBST containing 3% dried milk (block buffer) for 1 h. Diluted serum samples were added to wells and incubated for 1 h. After washing with PBST, 1 mcg/ml sheep anti-mouse CD93 antibody (AF1696, R&D Systems) was added to wells for 1 h followed by incubation with anti-sheep horseradish peroxidase-conjugated streptavidin (Jackson, Immunoresearch, West Grove, PA) diluted 1:2,000 in 1% dried milk. .. Sigma fast o -phenylenediamine dihydrochloride peroxidase substrate (Sigma-Aldrich, St. Louis, MO) was added, and the change in absorbance at 405 nm was measured.

    Article Title: Screening for CD93 (C1qRp)-associated polymorphism(S) in the diagnosis, prevention and treatment of autoimmune diseases
    Article Snippet: .. Briefly, Immulon 2 HB plates (Thermo Labsystems, Franklin, Mass.) were coated overnight at 4° C. with 2 □g/ml rat monoclonal anti-mouse CD93 (mAB 1696, R&D Systems, Minneapolis, Minn.) in 0.1 M carbonate buffer, pH 7.4, washed with phosphate buffer saline (PBS) containing 0.05% Tween-20 (PBST), and blocked with PBST containing 3% dried milk (block buffer) for 1 hr. ..

    Incubation:

    Article Title: A novel CD93 polymorphism in non-obese diabetic (NOD) and NZB/W F1 mice is linked to a CD4+ iNKT cell deficient state
    Article Snippet: .. Briefly, Immulon 2 HB plates (Thermo Labsystems, Franklin, MA) were coated overnight at 4°C with 2 mcg/ml rat monoclonal anti-mouse CD93 (mAB1696, R&D Systems, Minneapolis, MN) in 0.1 M carbonate buffer, pH 7.4, washed with phosphate buffer saline containing 0.05% Tween-20 (PBST), and blocked with PBST containing 3% dried milk (block buffer) for 1 h. Diluted serum samples were added to wells and incubated for 1 h. After washing with PBST, 1 mcg/ml sheep anti-mouse CD93 antibody (AF1696, R&D Systems) was added to wells for 1 h followed by incubation with anti-sheep horseradish peroxidase-conjugated streptavidin (Jackson, Immunoresearch, West Grove, PA) diluted 1:2,000 in 1% dried milk. .. Sigma fast o -phenylenediamine dihydrochloride peroxidase substrate (Sigma-Aldrich, St. Louis, MO) was added, and the change in absorbance at 405 nm was measured.



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    Immunoglobulin heavy chain gene-sequencing of single GAC+ B cells depicted as Circos plots (See Experimental Procedures). (A) GAC+ B cells (n=94) sorted from naïve adult C57BL/6 mice, (B) Adult C57BL/6 mice were immunized with GAS at d56 and GAC+ B cells were sorted 14 days post-immunization (d.p.i.) (n=74), (C) GAC+ B cells sorted from d56 resting adult C57BL/6 mice previously immunized as pups at d14 (n=87). IGH sequences are pooled from naïve (n=4), d56-immunized (n=4) or d14-immunized (n=4) mice from one representative experiment completed twice. Ribbon color coresponds IGHV identity (IGHV6 blue, IGHV7–3 red, all other IGHVs grey), IGHJ usage and IGHV mutations are depicted by inlayed heatmaps. (D) CDRH3 length distribution of pooled IGH gene sequences of identifiied IGHV families of GAC+ B cells. (E) CDRH3 consensus logos of of the mode CDRH3 length for pooled IGHV6–3 and IGHV7–3 IGH gene sequences. See also Figure S2. (F) Relative IGHV gene abundance in recall responses elicited from adult and d14 GAS-immunized mice following a prime-boost regimen. See also Figure S3. (G) Relative abundance of IGHV gene utilization of GAC+ B cells in d14, d21 and d28 neonates, adult (8–10wk) mice and aged adult (10–12mo) mice. (H) Relative IGHV gene abundance in <t>AA4.1+</t> immature and AA4.1− mature GAC+ B cells isolated from d28 neonates. See also Figure S3.
    Rat Monoclonal Anti Mouse Cd93 Aa4.1, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Immunoglobulin heavy chain gene-sequencing of single GAC+ B cells depicted as Circos plots (See Experimental Procedures). (A) GAC+ B cells (n=94) sorted from naïve adult C57BL/6 mice, (B) Adult C57BL/6 mice were immunized with GAS at d56 and GAC+ B cells were sorted 14 days post-immunization (d.p.i.) (n=74), (C) GAC+ B cells sorted from d56 resting adult C57BL/6 mice previously immunized as pups at d14 (n=87). IGH sequences are pooled from naïve (n=4), d56-immunized (n=4) or d14-immunized (n=4) mice from one representative experiment completed twice. Ribbon color coresponds IGHV identity (IGHV6 blue, IGHV7–3 red, all other IGHVs grey), IGHJ usage and IGHV mutations are depicted by inlayed heatmaps. (D) CDRH3 length distribution of pooled IGH gene sequences of identifiied IGHV families of GAC+ B cells. (E) CDRH3 consensus logos of of the mode CDRH3 length for pooled IGHV6–3 and IGHV7–3 IGH gene sequences. See also Figure S2. (F) Relative IGHV gene abundance in recall responses elicited from adult and d14 GAS-immunized mice following a prime-boost regimen. See also Figure S3. (G) Relative abundance of IGHV gene utilization of GAC+ B cells in d14, d21 and d28 neonates, adult (8–10wk) mice and aged adult (10–12mo) mice. (H) Relative IGHV gene abundance in AA4.1+ immature and AA4.1− mature GAC+ B cells isolated from d28 neonates. See also Figure S3.

    Journal: Immunity

    Article Title: Neonatal exposure to commensal bacteria-derived antigens directs polysaccharide-specific B-1 B cell repertoire development

    doi: 10.1016/j.immuni.2020.06.006

    Figure Lengend Snippet: Immunoglobulin heavy chain gene-sequencing of single GAC+ B cells depicted as Circos plots (See Experimental Procedures). (A) GAC+ B cells (n=94) sorted from naïve adult C57BL/6 mice, (B) Adult C57BL/6 mice were immunized with GAS at d56 and GAC+ B cells were sorted 14 days post-immunization (d.p.i.) (n=74), (C) GAC+ B cells sorted from d56 resting adult C57BL/6 mice previously immunized as pups at d14 (n=87). IGH sequences are pooled from naïve (n=4), d56-immunized (n=4) or d14-immunized (n=4) mice from one representative experiment completed twice. Ribbon color coresponds IGHV identity (IGHV6 blue, IGHV7–3 red, all other IGHVs grey), IGHJ usage and IGHV mutations are depicted by inlayed heatmaps. (D) CDRH3 length distribution of pooled IGH gene sequences of identifiied IGHV families of GAC+ B cells. (E) CDRH3 consensus logos of of the mode CDRH3 length for pooled IGHV6–3 and IGHV7–3 IGH gene sequences. See also Figure S2. (F) Relative IGHV gene abundance in recall responses elicited from adult and d14 GAS-immunized mice following a prime-boost regimen. See also Figure S3. (G) Relative abundance of IGHV gene utilization of GAC+ B cells in d14, d21 and d28 neonates, adult (8–10wk) mice and aged adult (10–12mo) mice. (H) Relative IGHV gene abundance in AA4.1+ immature and AA4.1− mature GAC+ B cells isolated from d28 neonates. See also Figure S3.

    Article Snippet: Rat monoclonal anti-Mouse CD93 (clone AA4.1), FITC , BD Biosciences , Cat: 559156.

    Techniques: Sequencing, Isolation

    KEY RESOURCES TABLE

    Journal: Immunity

    Article Title: Neonatal exposure to commensal bacteria-derived antigens directs polysaccharide-specific B-1 B cell repertoire development

    doi: 10.1016/j.immuni.2020.06.006

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Rat monoclonal anti-Mouse CD93 (clone AA4.1), FITC , BD Biosciences , Cat: 559156.

    Techniques: Recombinant, Sequencing, Software

    Journal: eLife

    Article Title: Plasmodium -specific atypical memory B cells are short-lived activated B cells

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    Figure Lengend Snippet:

    Article Snippet: Antibody , Monoclonal Rat anti-Mouse CD93 (AA4.1) , eBioscience , 17–5892 , (dil 1/100).

    Techniques: Produced, Activation Assay, Recombinant, Knock-In, Construct, Purification, Sequencing, Staining, Adjuvant, Software